Based on the investigation of the technological innovation process of QL Pharmaceutical， it adopted the longitudinal single case study method， based on the 6C research framework， to explore the dynamic and internal mechanism of the evolution of enterprise innovation ecosystem. It finds that the evolution of QL Pharmaceutical innovation ecosystem has experienced two stages： emerging and diversification. In the emerging stage， the ecosystem has fewer members， loose structure and poor openness， and technological innovation focuses on value acquisition. In the diversification stage， the members of the innovation ecosystem increase， the structure is perfect， the openness is strong， and the technical innovation focuses on value creation. In different stages of innovation ecosystem evolution， the core leaders change dynamically， and the behavior and mechanism of different leaders are different. The construction of enterprises enabling capacity and empowerment capability jointly promotes the sustainable evolution and upgrading of the enterprise innovation ecosystem. Based on this， it opens the black box of the motivation and mechanism of innovation ecosystem evolution， which has certain guiding value for the construction of innovation ecosystem of enterprises.