Based on resource dependence theory and knowledge combination theory， it examined the influence mechanism of knowledge search of center enterprises on value co-creation in innovation ecosystem from two dimensions of interaction and non-interaction， and explored the mediating role of knowledge recombination and the moderating role of knowledge potential difference. The results on the research questionnaire of 364 high-technology enterprises show that both interactive and non-interactive knowledge search positively influence value co-creation； knowledge recombination plays a mediating role in the relationship between them； knowledge potential difference positively moderates the relationship between knowledge recombination and value co-creation，and the mediating effect of knowledge recombination on the relationship between knowledge search and value co-creation. Based on the participant level and from the perspective of knowledge flow， it analyzes value co-creation mechanism in innovation ecosystem， expands the research on the antecedents of value co-creation in innovation ecosystem， and provides theoretical basis and practical enlightenment for the innovation ecosystem center enterprises to choose appropriate knowledge search strategies and improve innovation ability and realize value co-creation.