Using the panel data of manufacturing enterprises and new infrastructure from 2013 to 2019， it explored the impact mechanism of new infrastructure on enterprise green technology innovation and the moderating effect of industrial agglomeration. The results are as following. At present， a significant positive effect of a large amount of investment in information infrastructure construction on the ‘quantity and quality’ of green technology innovation is identified on the whole， especially for high-quality green technology innovation， which is supported by the test based on the threshold of manufacturing industry agglomeration level. However， the congestion effect of industrial agglomeration hinders this promotion process. In general， integrated infrastructure investment has a significant role in promoting the ‘quantity and quality’ of green technology innovation of enterprises， but it is quite different in different regions and at the different level of industrialization. The integration of infrastructure has significantly improved green technology innovation capability of enterprises in eastern China and provinces with higher industrialization， but has not yet played a role in promoting the enterprises in central and western China and provinces with lower industrialization. The scale effect of industrial clusters offsets the positive impact of some integrated infrastructure on green technology innovation of enterprises in provinces with higher industrialization. During the sample period， hastily increasing investment in innovation infrastructure construction is not conducive to the ‘simultaneous improvement of quantity and quality’ of green technology innovation. But the diffusion effect of industrial agglomeration alleviates this adverse effect on the whole. Then， when the industrial agglomeration level of manufacturing industry crosses the inflection point of 1.335， increasing investment in innovation infrastructure construction greatly promotes high-quality green technology innovation of enterprises. The conclusions provide reference suggestions for the key planning and layout of new infrastructure investment types， and effectively create a good environment for the green transformation of enterprises， which is aimed at promoting China’s industrial structure upgrading.